WebThe determination of creatinine has been developed using Jaffé reaction between the creatinine and picric acid in alkaline condition. The alkaline picrate color on μ PAD changed from yellow to orange in the presence of creatinine and the orange intensity is directly proportional to the creatinine amount in a linearity range of 0.20-6.0 mM as a detection … WebParticular interest is attached to a new reaction for creatinine because the Jaffe reaction with picric acid has hitherto been the only known highly sensitive reaction for ... Reacting compound 5 -0.35 0.34 0.4 0.5 0.36 0.43 0.45 0.53 10 1 With ... as in the creatine …
Mechanism of interference with the Jaffé reaction for creatinine ...
WebThe assay is based on the reaction of creatinine with sodium picrate as described by Jaffé. Creatinine reacts with alkaline picrate forming a red complex. The time interval chosen for measurements avoids interferences from other serum constituents. The intensity of the color formed is proportional to the creatinine WebThe Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine. In 1886, Max Jaffe (1841–1911) wrote about its basic principles in the paper Über den Niederschlag, welchen Pikrinsäure in normalem Harn … jethalal height
Disposable Nonenzymatic Uric Acid and Creatinine Sensors ... - Hindawi
WebIn experiments described in a previous publication (I), it was found that both the creatinine and the picric acid involved in Jaffe’s reaction could, after acidification with acetic acid, be recovered unchanged. It was also found that, when a fairly concentrated solution of creatinine, sodium picrate, and sodium hydroxide is acidified with hydrochloric acid, there … WebIn the Jaffe reaction, a red-orange chromogen is formed when creatinine reacts with Picric acid Substances known to increase results when measuring creatinine by the Jaffe reaction include all of the following except ascorbic acid bilirubin glucose alpha- keto acids WebThe normal serum creatinine (sCr) varies with the subject's body muscle mass and with the technique used to measure it. For the adult male, the normal range is 0.6 to 1.2 mg/dl, or 53 to 106 μmol/L by the kinetic or enzymatic method, and 0.8 to 1.5 mg/dl, or 70 to 133 … jethalal monthly income